Professional Industrial Valves Manufacturer

Liquid chromatograph sample cleaning method of the valve

by:Mech     2020-08-21
Sampling Industrial Valves switch to the sample position ( Inject) , want to be in this position for a period of time, the mobile phase fully flush quantitative tube samples, so that we can ensure that there is a higher accuracy, and don't have to wash the sample tube fixed quantity. This is controlled by chromatographic instrument washing obviously better than manual washing. Note: rinse need at least for the mobile phase volume into 10 times the size of the sample. For example, if the samples from 20 to 100 mu mu L sample L quantitative tube, and the mobile phase flow rate of 1 ml/min at this time, you need the sample position at least 12 s, namely: 20 mu mu L x 10/1000 l. min - 1 = 0. 2min=12s。 When sample quantitative tube after adequate flushing, turning the handle can be turned back to the sampling position ( 负载) , also can continue to maintain in the sampling position, switch back to the sampling position until the next sampling. When switch back to the sampling position, the sample or trace sample into the injector needle pulled out from the injection valve. To prevent cross contamination, normally don't have to wash after each sample injection Industrial Valves needle into the mouth. Sample according to the design of patent in the Industrial Valves hole connected directly into the samples, can be directly connected to the needles the front end of the sample tube at the end of the quantitative, there is no other space for sample residue. So the next time the sample, there won't be the last remaining quantitative tube samples into the sample. But in the process of injection needles insert or pull out, there will be a trace of sample deposited in needle seating area. Precision of the measurement shows that the residual of nl 1 ~ 10 nl. Suggesting that into 20 mu L samples, will remain zero. 005% ~ 0. 05%. Flush the needle into the mouth after each sample you can use this residual is rinsed clean. Rinse mouth needle into the process of set for: mobile phase velocity is zero. 1 ml/min ~ 1 ml/min, import the needle mouth rinse head ( Rheodyne part number 7125 - 054). Connected to a relatively large volume injection, with a large number of mobile phase in the sample position cleaning injection needle into the mouth. Such of the liquid around the sample into the injection valve tube is being exhausted from port sample overflow tube # 5. Import this process can be needle mouth, import guide tube, needle tube and needle seal thoroughly clean. And syringe completely plug-in flushing method, are not all clean the surface of the part. Import in the sampling position insert the needle needle, needle push needle seal within a small amount of sample liquid ( The last time the import pipe flushing needle left) Quantitative tube into the sample. When the sample liquid and the different composition of mobile phase, and at the same time using some full of quantitative tube sample way, on the high sensitivity of the detector may appear strange peak. So had better mobile phase when flushing needle rinse. If has been found to have serious cross contamination phenomenon, check for the following reasons: 1. Using a needle length is not enough, The length of the needle must be at least 5 cm) , the end of the needle can not reach the surface of the stator. ⅱ。 The needle is not fully insert the needle into the mouth. ⅲ。 Needle pollutants or needle seal wear down the import the mouths of shavings, needles end cannot contact surface of the stator. If not considering cross contamination, 10 or 20 times per sample import best wash needle mouth, so that we can ensure the needle import tube filled with fluid. In the process of needle insert or pull out, clean needles or dilution of samples of pollution in the region at the same time also make needle import overflow mouth and sample # 5 mouth filled with fluid, so as to prevent the air into the tube sample.
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